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NDB ID: NA2910    PDB ID: 4OO8 


Title:

CRYSTAL STRUCTURE OF STREPTOCOCCUS PYOGENES CAS9 IN COMPLEX WITH GUIDE RNA AND TARGET DNA

Molecular Description:

CRISPR-associated endonuclease Cas9/Csn1 (E.C.3.1.-.-)

Deposited:

2014-01-31

Released:

2014-02-26

Structural Keywords:

RH DOUBLE HELIX, HAIRPIN LOOP, INTERNAL LOOP, FLIPPED OUT BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Nishimasu, H., Ran, F.A., Hsu, P.D., Konermann, S., Shehata, S.I., Dohmae, N., Ishitani, R., Zhang, F., Nureki, O.
Crystal structure of Cas9 in complex with guide RNA and target DNA 
Cell(Cambridge,Mass.), 156, pp. 935 - 949, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 76.713   b = 105.694   c = 126.817 (Ångstroms)

α = 97.68   β = 98.43   γ = 100.31 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2235 for 130396 reflections in the resolution range 46.701 to 2.5 Ångstroms with Fobs >  1.98 sigma(Fobs) and with I >  0.0 sigma(I)