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NDB ID: NA2982    PDB ID: 4Q5S 


Title:

THERMUS THERMOPHILUS RNA POLYMERASE INITIALLY TRANSCRIBING COMPLEX CONTAINING 6-MER RNA

Molecular Description:

DNA-directed RNA polymerase subunit alpha (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit beta' (E.C.2.7.7.6)
DNA-directed RNA polymerase subunit omega (E.C.2.7.7.6)
RNA polymerase sigma factor SigA
rna
dna

Deposited:

2014-04-17

Released:

2014-07-09

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Basu, R.S., Warner, B.A., Molodtsov, V., Pupov, D., Esyunina, D., Fernandez-Tornero, C., Kulbachinskiy, A., Murakami, K.S.
Structural basis of transcription initiation by bacterial RNA polymerase holoenzyme. 
J.Biol.Chem., 289, pp. 24549 - 24559, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 187.089   b = 102.075   c = 297.263 (Ångstroms)

α = 90.0   β = 98.14   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2698 for 106154 reflections in the resolution range 41.977 to 3.0 Ångstroms with Fobs >  1.38 sigma(Fobs) and with I >  0.0 sigma(I)