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NDB ID: NA3069    PDB ID: 4UN4 


Title:

CRYSTAL STRUCTURE OF CAS9 BOUND TO PAM-CONTAINING DNA TARGET WITH MISMATCHES AT POSITIONS 1-2

Molecular Description:

CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1/RNA Complex

Deposited:

2014-05-25

Released:

2014-07-23

Structural Keywords:

A DOUBLE HELIX, HAIRPIN LOOP, INTERNAL LOOP

Nucleic Acid Sequence:

Click to show/hide 4 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Anders, C., Niewoehner, O., Duerst, A., Jinek, M.
Structural Basis of Pam-Dependent Target DNA Recognition by the Cas9 Endonuclease 
Nature, 513, pp. 569 - , 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 177.78   b = 66.816   c = 186.834 (Ångstroms)

α = 90.0   β = 111.39   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2185 for 160249 reflections in the resolution range 47.493 to 2.371 Ångstroms with Fobs >  1.3 sigma(Fobs) and with I >  0.0 sigma(I)