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NDB ID: NA3085    PDB ID: 4QYZ 


Title:

CRYSTAL STRUCTURE OF A CRISPR RNA-GUIDED SURVEILLANCE COMPLEX, CASCADE, BOUND TO A SSDNA TARGET

Molecular Description:

CRISPR system Cascade subunit CasA
CRISPR system Cascade subunit CasB
CRISPR system Cascade subunit CasC
CRISPR system Cascade subunit CasD
CRISPR system Cascade subunit CasE (E.C.3.1.-.-)

Deposited:

2014-07-26

Released:

2014-09-03

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Mulepati, S., Heroux, A., Bailey, S.
Structural biology. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target. 
Science, 345, pp. 1479 - 1484, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31 2 1

Cell Constants:

a = 223.869   b = 223.869   c = 290.651 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2257 for 156727 reflections in the resolution range 39.473 to 3.0303 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)