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NDB ID: PD0066    PDB ID: 1QSS 


Title:

DDGTP-TRAPPED CLOSED TERNARY COMPLEX OF THE LARGE FRAGMENT OF DNA POLYMERASE I FROM THERMUS AQUATICUS

Molecular Description:

KLENOW FRAGMENT OF DNA POLYMERASE I (E.C.2.7.7.7)/DNA COMPLEX

Deposited:

1999-06-23

Released:

1999-08-16

Structural Keywords:

B DOUBLE HELIX, FLIPPED-OUT BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Li, Y., Mitaxov, V., Waksman, G.
Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation. 
Proc.Natl.Acad.Sci.USA, 96, pp. 9491 - 9496, 1999.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31 2 1

Cell Constants:

a = 108.557   b = 108.557   c = 90.527 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.228 for 26114 reflections in the resolution range 30.0 to 2.3 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)