A Portal for Three-dimensional Structural Information about Nucleic Acids
As of 14-Oct-2020 number of released structures: 11024
Search for released structures

NDB ID: PD0085    PDB ID: 1DC1 


Title:

RESTRICTION ENZYME BSOBI/DNA COMPLEX STRUCTURE: ENCIRCLEMENT OF THE DNA AND HISTIDINE-CATALYZED HYDROLYSIS WITHIN A CANONICAL RESTRICTION ENZYME FOLD

Molecular Description:

BSOBI RESTRICTION ENDONUCLEASE (E.C.3.1.21.4)/DNA COMPLEX

Deposited:

1999-11-04

Released:

2001-02-21

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

van der Woerd, M.J., Pelletier, J.J., Xu, S., Friedman, A.M.
Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis. 
Structure, 9, pp. 133 - 144, 2001.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 80.294   b = 87.823   c = 99.454 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the X-PLOR program. The R value is 0.19 for 75657 reflections in the resolution range 8.0 to 1.7 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)