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NDB ID: PD0101    PDB ID: 1DFM 


Title:

CRYSTAL STRUCTURE OF RESTRICTION ENDONUCLEASE BGLII COMPLEXED WITH DNA 16-MER

Molecular Description:

ENDONUCLEASE BGLII/DNA COMPLEX

Deposited:

1999-12-06

Released:

2000-02-21

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Lukacs, C.M., Kucera, R., Schildkraut, I., Aggarwal, A.K.
Understanding the immutability of restriction enzymes: crystal structure of BglII and its DNA substrate at 1.5 A resolution. 
Nat.Struct.Biol., 7, pp. 134 - 140, 2000.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 48.7   b = 101.8   c = 116.9 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.192 for 87061 reflections in the resolution range 20.0 to 1.5 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  1.0 sigma(I)