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NDB ID: PD0129    PDB ID: 1EO3 


Title:

INHIBITION OF ECORV ENDONUCLEASE BY DEOXYRIBO-3'-S-PHOSPHOROTHIOLATES: A HIGH RESOLUTION X-RAY CRYSTALLOGRAPHIC STUDY

Molecular Description:

TYPE II RESTRICTION ENZYME ECORV (E.C.3.1.21.4)/DNA COMPLEX

Deposited:

2000-03-21

Released:

2000-04-04

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Horton, N.C., Connolly, B.A., Perona, J.J.
Inhibition of EcoRV Endonuclease by Deoxyribo-3'-S-phosphorothiolates: A High-Resolution X-ray Crystallographic Study 
J.Am.Chem.Soc., 122, pp. 3314 - 3324, 2000.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 48.0   b = 48.5   c = 63.8 (Ångstroms)

α = 97.0   β = 108.9   γ = 106.3 (degrees)

Refinement:

The structure was refined using the X-PLOR program. The R value is 0.2 for 29583 reflections in the resolution range 4.9 to 2.0 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  1.0 sigma(I)