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NDB ID: PD0131    PDB ID: 1EON 


Title:

ECORV BOUND TO 3'-S-PHOSPHOROTHIOLATE DNA AND CA2+

Molecular Description:

TYPE II RESTRICTION ENZYME ECORV (E.C.3.1.21.4)/DNA COMPLEX

Deposited:

2000-03-23

Released:

2000-04-04

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Horton, N.C., Connolly, B.A., Perona, J.J.
Inhibition of EcoRV Endonuclease by Deoxyribo-3'-S-phosphorothiolates: A High-Resolution X-ray Crystallographic Study 
J.Am.Chem.Soc., 122, pp. 3314 - 3324, 2000.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 47.9   b = 48.6   c = 63.9 (Ångstroms)

α = 96.9   β = 108.9   γ = 106.8 (degrees)

Refinement:

The structure was refined using the X-PLOR program. The R value is 0.23 for 61223 reflections in the resolution range 4.8 to 1.6 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  1.0 sigma(I)