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NDB ID: PD0140    PDB ID: 1EVW 


Title:

L116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA.

Molecular Description:

Deposited:

2000-04-20

Released:

2000-08-03

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 4 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Galburt, E.A., Chadsey, M.S., Jurica, M.S., Chevalier, B.S., Erho, D., Tang, W., Monnat Jr., R.J., Stoddard, B.L.
Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site. 
J.Mol.Biol., 300, pp. 877 - 887, 2000.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 182.0   b = 73.1   c = 92.7 (Ångstroms)

α = 90.0   β = 95.4   γ = 90.0 (degrees)

Refinement:

The structure was refined using the X-PLOR program. The R value is 0.0 for 19707 reflections in the resolution range 50.0 to 3.1 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)