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NDB ID: PD0152    PDB ID: 1D1U 


Title:

USE OF AN N-TERMINAL FRAGMENT FROM MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE TO FACILITATE CRYSTALLIZATION AND ANALYSIS OF A PSEUDO-16-MER DNA MOLECULE CONTAINING G-A MISPAIRS

Molecular Description:

PROTEIN (MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE)/DNA COMPLEX

Deposited:

1999-09-21

Released:

2000-04-02

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Cote, M.L., Yohannan, S.J., Georgiadis, M.M.
Use of an N-terminal fragment from moloney murine leukemia virus reverse transcriptase to facilitate crystallization and analysis of a pseudo-16-mer DNA molecule containing G-A mispairs. 
Acta Crystallogr.,Sect.D, 56, pp. 1120 - 1131, 2000.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 54.738   b = 145.491   c = 46.737 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.23 for 17117 reflections in the resolution range 50.0 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)