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NDB ID: PD0256    PDB ID: 1K4T 


Title:

HUMAN DNA TOPOISOMERASE I (70 KDA) IN COMPLEX WITH THE POISON TOPOTECAN AND COVALENT COMPLEX WITH A 22 BASE PAIR DNA DUPLEX

Molecular Description:

DNA TOPOISOMERASE I (E.C.5.99.1.2)/DNA Complex

Deposited:

2001-10-08

Released:

2002-12-04

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Staker, B.L., Hjerrild, K., Feese, M.D., Behnke, C.A., Burgin Jr., A.B., Stewart, L.J.
The mechanism of topoisomerase I poisoning by a camptothecin analog 
Proc.Natl.Acad.Sci.USA, 99, pp. 15387 - 15392, 2002.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 57.093   b = 116.26   c = 75.217 (Ångstroms)

α = 90.0   β = 94.16   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNX program. The R value is 0.0 for 45727 reflections in the resolution range 19.89 to 2.1 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)