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NDB ID: PD0302    PDB ID: 1L3U 


Title:

CRYSTAL STRUCTURE OF BACILLUS DNA POLYMERASE I FRAGMENT PRODUCT COMPLEX WITH 11 BASE PAIRS OF DUPLEX DNA FOLLOWING ADDITION OF A DTTP AND A DATP RESIDUE.

Molecular Description:

DNA POLYMERASE I (E.C.2.7.7.7)/DNA Complex

Deposited:

2002-03-01

Released:

2003-03-25

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Johnson, S.J., Taylor, J.S., Beese, L.S.
Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations 
Proc.Natl.Acad.Sci.USA, 100, pp. 3895 - 3900, 2003.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 86.429   b = 93.018   c = 106.137 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.1959 for 63346 reflections in the resolution range 46.09 to 1.8 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)