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NDB ID: PD0335    PDB ID: 1M5X 


Title:

CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE I-MSOI BOUND TO ITS DNA SUBSTRATE

Molecular Description:

DNA endonuclease I-MsoI/DNA Complex

Deposited:

2002-07-10

Released:

2003-06-03

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Chevalier, B., Turmel, M., Lemieux, C., Monnat, R.J., Stoddard, B.L.
Flexible DNA Target Site Recognition by Divergent Homing Endonuclease Isoschizomers I-CreI and I-MsoI 
J.Mol.Biol., 329, pp. 253 - 269, 2003.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 41.51   b = 42.21   c = 71.32 (Ångstroms)

α = 73.34   β = 73.17   γ = 71.09 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.0 for 19849 reflections in the resolution range 19.96 to 2.25 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)