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NDB ID: PD0337    PDB ID: 1MA7 


Title:

CRYSTAL STRUCTURE OF CRE SITE-SPECIFIC RECOMBINASE COMPLEXED WITH A MUTANT DNA SUBSTRATE, LOXP-A8/T27

Molecular Description:

CRE RECOMBINASE/DNA Complex

Deposited:

2002-08-01

Released:

2003-07-08

Structural Keywords:

B DOUBLE HELIX, HOLLIDAY JUNCTION

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Martin, S.S., Chu, V.C., Baldwin, E.P.
Modulation of the active complex assembly and turnover rate by protein-DNA interactions in Cre-LoxP recombination 
Biochemistry, 42, pp. 6814 - 6826, 2003.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 107.54   b = 122.24   c = 180.03 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the TNT program. The R value is 0.0 for 51364 reflections in the resolution range 5.0 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)