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NDB ID: PD0345    PDB ID: 1MSW 


Title:

STRUCTURAL BASIS FOR THE TRANSITION FROM INITIATION TO ELONGATION TRANSCRIPTION IN T7 RNA POLYMERASE

Molecular Description:

DNA-directed RNA polymerase (E.C.2.7.7.6)/DNA/RNA Complex

Structural Keywords:

RH DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Yin, Y.W., Steitz, T.A.
Structural Basis for the Transition from Initiation to Elongation Transcription in T7 RNA Polymerase 
Science, 298, pp. 1387 - 1395, 2002.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 142.91   b = 145.46   c = 145.6 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.241 for 61181 reflections in the resolution range 20.0 to 2.1 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)