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NDB ID: PD0381    PDB ID: 1NK8 


Title:

A BACILLUS DNA POLYMERASE I PRODUCT COMPLEX BOUND TO A GUANINE-THYMINE MISMATCH AFTER A SINGLE ROUND OF PRIMER EXTENSION, FOLLOWING INCORPORATION OF DCTP.

Molecular Description:

DNA polymerase I (E.C.2.7.7.7)/DNA Complex

Deposited:

2003-01-02

Released:

2004-03-30

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASE

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Johnson, S.J., Beese, L.S.
Structures of mismatch replication errors observed in a DNA polymerase. 
Cell(Cambridge,Mass.), 116, pp. 803 - 816, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 87.723   b = 93.579   c = 105.279 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.207 for 60471 reflections in the resolution range 26.32 to 1.9 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)