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NDB ID: PD0406    PDB ID: 1OWF 


Title:

CRYSTAL STRUCTURE OF A MUTANT IHF (BETAE44A) COMPLEXED WITH THE NATIVE H' SITE

Molecular Description:

Integration Host Factor Alpha-subunit/Integration Host Factor Beta-subunit/Phage lambda H' site/5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3'/5'-D(*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP*CP*C)-3'

Deposited:

2003-03-28

Released:

2003-07-15

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES, DNA BENDING

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Lynch, T.W., Read, E.K., Mattis, A.N., Gardner, J.F., Rice, P.A.
Integration Host Factor: putting a twist on protein-DNA recognition 
J.Mol.Biol., 330, pp. 493 - 502, 2003.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 46.66   b = 58.832   c = 181.314 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.232 for 26109 reflections in the resolution range 42.78 to 1.95 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)