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NDB ID: PD0463    PDB ID: 1Q3V 


Title:

CRYSTAL STRUCTURE OF A WILD-TYPE CRE RECOMBINASE-LOXP SYNAPSE: PHOSPHOTYROSINE COVALENT INTERMEDIATE

Molecular Description:

Cre recombinase/DNA COMPLEX

Deposited:

2003-08-01

Released:

2003-09-16

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Ennifar, E., Meyer, J.E.W., Buchholz, F., Stewart, A.F., Suck, D.
Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation 
Nucleic Acids Res., 31, pp. 5449 - 5460, 2003.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 108.938   b = 164.16   c = 194.759 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.2471 for 70849 reflections in the resolution range 29.92 to 2.91 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)