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NDB ID: PD0498    PDB ID: 1RR8 


Title:

STRUCTURAL MECHANISMS OF CAMPTOTHECIN RESISTANCE BY MUTATIONS IN HUMAN TOPOISOMERASE I

Molecular Description:

DNA Topoisomerase I/DNA
(S)-10-[(DIMETHYLAMINO)METHYL]-4-ETHYL-4
9-DIHYDROXY-1H-PYRANO[3'
4':6
7]INOLIZINO[1
2-B]-QUINOLINE-3
14(4H
12H)-DIONE

Deposited:

2003-12-08

Released:

2004-07-06

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Chrencik, J.E., Staker, B.L., Burgin, A.B., Pourquier, P., Pommier, Y., Stewart, L., Redinbo, M.R.
Mechanisms of camptothecin resistance by human topoisomerase I mutations 
J.Mol.Biol., 339, pp. 773 - 784, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 57.59   b = 115.585   c = 75.01 (Ångstroms)

α = 90.0   β = 96.29   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.292 for 27855 reflections in the resolution range 19.7 to 2.6 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)