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NDB ID: PD0515    PDB ID: 1SA3 


Title:

AN ASYMMETRIC COMPLEX OF RESTRICTION ENDONUCLEASE MSPI ON ITS PALINDROMIC DNA RECOGNITION SITE

Molecular Description:

Type II restriction enzyme MspI (E.C.3.1.21.4)/DNA Complex

Deposited:

2004-02-06

Released:

2004-09-07

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Xu, Q.S., Kucera, R.B., Roberts, R.J., Guo, H.C.
An Asymmetric Complex of Restriction Endonuclease MspI on Its Palindromic DNA Recognition Site. 
STRUCTURE, 12, pp. 1741 - 1747, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 50.193   b = 131.601   c = 59.318 (Ångstroms)

α = 90.0   β = 109.69   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.225 for 51260 reflections in the resolution range 42.58 to 1.95 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)