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NDB ID: PD0516    PDB ID: 1S97 


Title:

DPO4 WITH GT MISMATCH

Molecular Description:

DNA polymerase IV/DNA

Deposited:

2004-02-03

Released:

2004-04-27

Structural Keywords:

A DOUBLE HELIX, FLIPPED-OUT BASES, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Trincao, J., Johnson, R.E., Wolfle, W.T., Escalante, C.R., Prakash, S., Prakash, L., Aggarwal, A.K.
Dpo4 is hindered in extending a G.T mismatch by a reverse wobble 
Nat.Struct.Mol.Biol., 11, pp. 457 - 462, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 104.433   b = 100.644   c = 110.426 (Ångstroms)

α = 90.0   β = 94.89   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.238 for 76508 reflections in the resolution range 29.64 to 2.4 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)