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NDB ID: PD0527    PDB ID: 1SL2 


Title:

TERNARY 5' COMPLEX OF T7 DNA POLYMERASE WITH A DNA PRIMER/TEMPLATE CONTAINING A CIS-SYN THYMINE DIMER ON THE TEMPLATE AND AN INCOMING NUCLEOTIDE

Molecular Description:

DNA POLYMERASE (E.C.2.7.7.7)/THIOREDOXIN 1/DNA Complex

Deposited:

2004-03-05

Released:

2004-07-06

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASE

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Li, Y., Dutta, S., Doublie, S., Bdour, H.M., Taylor, J.S., Ellenberger, T.
Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7. 
Nat.Struct.Mol.Biol., 11, pp. 784 - 790, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 104.983   b = 212.599   c = 51.945 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.246 for 48921 reflections in the resolution range 50.0 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)