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NDB ID: PD0549    PDB ID: 1T8I 


Title:

HUMAN DNA TOPOISOMERASE I (70 KDA) IN COMPLEX WITH THE POISON CAMPTOTHECIN AND COVALENT COMPLEX WITH A 22 BASE PAIR DNA DUPLEX

Molecular Description:

DNA Topoisomerase I/ 5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*T)-3'

Deposited:

2004-05-12

Released:

2005-05-31

Structural Keywords:

B DOUBLE HELIX, BASE INTERCALATED

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Staker, B.L., Feese, M.D., Cushman, M., Pommier, Y., Zembower, D., Stewart, L., Burgin, A.B.
Structures of three classes of anticancer agents bound to the human topoisomerase I-DNA covalent complex 
J.Med.Chem., 48, pp. 2336 - 2345, 2005.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 57.363   b = 114.423   c = 74.118 (Ångstroms)

α = 90.0   β = 93.49   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.24356 for 16492 reflections in the resolution range 20.0 to 3.0 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)