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NDB ID: PD0554    PDB ID: 1TK0 


Title:

T7 DNA POLYMERASE TERNARY COMPLEX WITH 8 OXO GUANOSINE AND DDCTP AT THE INSERTION SITE

Molecular Description:

DNA POLYMERASE (E.C.2.7.7.7)/THIOREDOXIN 1/DNA Complex

Deposited:

2004-06-07

Released:

2004-08-31

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Brieba, L.G., Eichman, B.F., Kokoska, R.J., Doublie, S., Kunkel, T.A., Ellenberger, T.
Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase. 
Embo J., 23, pp. 3452 - 3461, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 106.23   b = 215.096   c = 52.136 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.0 for 50921 reflections in the resolution range 35.85 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)