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NDB ID: PD0586    PDB ID: 1UA1 


Title:

STRUCTURE OF AMINOFLUORENE ADDUCT PAIRED OPPOSITE CYTOSINE AT THE POLYMERASE ACTIVE SITE.

Molecular Description:

DNA polymerase I (E.C.2.7.7.7)/DNA Complex

Deposited:

2004-08-11

Released:

2004-09-28

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Hsu, G.W., Kiefer, J.R., Becherel, O.J., Fuchs, R.P.P., Beese, L.S.
Observing translesion synthesis of an aromatic amine DNA adduct by a high-fidelity DNA polymerase 
J.Biol.Chem., 279, pp. 50280 - 50285, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 87.639   b = 93.441   c = 105.465 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.2161 for 56558 reflections in the resolution range 33.7 to 2.0 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)