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NDB ID: PD0591    PDB ID: 1X9W 


Title:

T7 DNA POLYMERASE IN COMPLEX WITH A PRIMER/TEMPLATE DNA CONTAINING A DISORDERED N-2 AMINOFLUORENE ON THE TEMPLATE, CRYSTALLIZED WITH DIDEOXY-ATP AS THE INCOMING NUCLEOTIDE.

Molecular Description:

DNA polymerase (E.C.2.7.7.7)/Thioredoxin 1/DNA Complex

Deposited:

2004-08-24

Released:

2004-10-26

Structural Keywords:

RH DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Dutta, S., Li, Y., Johnson, D., Dzantiev, L., Richardson, C.C., Romano, L.J., Ellenberger, T.
Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis. 
Proc.Natl.Acad.Sci.Usa, 101, pp. 16186 - 16191, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 105.944   b = 213.559   c = 52.168 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.23 for 50543 reflections in the resolution range 47.68 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)