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NDB ID: PD0592    PDB ID: 1XC8     NAKB(beta): PD0592 


Title:

CRYSTAL STRUCTURE COMPLEX BETWEEN THE WILD-TYPE LACTOCOCCUS LACTIS FPG (MUTM) AND A FAPY-DG CONTAINING DNA

Molecular Description:

Formamidopyrimidine-DNA glycosylase (E.C.3.2.2.23)/DNA complex

Deposited:

2004-09-01

Released:

2005-09-06

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Coste, F., Ober, M., Carell, T., Boiteux, S., Zelwer, C., Castaing, B.
Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylase. 
J.Biol.Chem., 279, pp. 44074 - 44083, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 41 21 2

Cell Constants:

a = 92.513   b = 92.513   c = 142.867 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.17805 for 39561 reflections in the resolution range 17.247 to 1.95 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)