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NDB ID: PD0686    PDB ID: 1ZYQ 


Title:

T7 DNA POLYMERASE IN COMPLEX WITH 8OG AND INCOMING DDATP

Molecular Description:

/DNA POLYMERASE (E.C.2.7.7.7)/THIOREDOXIN 1/DNA Complex

Deposited:

2005-06-10

Released:

2005-11-22

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Brieba, L.G., Kokoska, R.J., Bebenek, K., Kunkel, T.A., Ellenberger, T.
A lysine residue in the fingers subdomain of t7 DNA polymerase modulates the miscoding potential of 8-oxo-7,8-dihydroguanosine. 
Structure, 13, pp. 1653 - 1659, 2005.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 106.148   b = 213.007   c = 52.99 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.224 for 32113 reflections in the resolution range 47.6 to 2.7 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)