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NDB ID: PD0812    PDB ID: 2GB7 


Title:

METAL-DEPLETED ECL18KI IN COMPLEX WITH UNCLEAVED, MODIFIED DNA

Molecular Description:

R.Ecl18kI/DNA Complex

Deposited:

2006-03-10

Released:

2006-06-20

Structural Keywords:

B DOUBLE HELIX, FLIPPED-OUT BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Bochtler, M., Szczepanowski, R.H., Tamulaitis, G., Grazulis, S., Czapinska, H., Manakova, E., Siksnys, V.
Nucleotide flips determine the specificity of the Ecl18kI restriction endonuclease 
Embo J., 25, pp. 2219 - 2229, 2006.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 77.697   b = 96.739   c = 192.012 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.208 for 157798 reflections in the resolution range 20.0 to 1.7 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)