NDB ID: PD0867    PDB ID: 2IBS 

---

Title:

CRYSTAL STRUCTURE OF THE ADENINE-SPECIFIC DNA METHYLTRANSFERASE M.TAQI COMPLEXED WITH THE COFACTOR ANALOG AETA AND A 10 BP DNA CONTAINING 2-AMINOPURINE AT THE TARGET POSITION

Molecular Description:

Modification methylase TaqI/DNA Complex

Deposited:

2006-09-12

Released:

2007-05-29

Structural Keywords:

B DOUBLE HELIX, FLIPPED-OUT BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Lenz, T., Bonnist, E.Y.M., Pljevaljcic, G., Neely, R.K., Dryden, D.T.F., Scheidig, A.J., Jones, A.C., Weinhold, E.
2-Aminopurine Flipped into the Active Site of the Adenine-Specific DNA Methyltransferase M.TaqI: Crystal Structures and Time-Resolved Fluorescence 
J.Am.Chem.Soc., 129, pp. 6240 - 6248, 2007.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 59.53   b = 69.16   c = 114.64 (Ångstroms)

α = 90.0   β = 92.18   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.19806 for 36299 reflections in the resolution range 19.67 to 2.4 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)

Download Data:

Asymmetric Unit coordinates (pdb format, Unix compressed(.gz))

Asymmetric Unit coordinates (cif format, Unix compressed(.gz))

Biological Assembly coordinates (pdb format) 1 2 

Structure Factors (cif format)

Coordinates (pdb format, Unix compressed(.gz))

Coordinates (cif format, Unix compressed(.gz))

NMR Restraints (cif format, Unix compressed(.gz))

XML | Complete with coordinates (xml format, GNU compressed(.gz))

XML | Coordinates only (xml format, GNU compressed(.gz))

XML | Header only (xml format, GNU compressed(.gz))