NDB ID: PD0887    PDB ID: 2ISP 

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Title:

TERNARY COMPLEX OF DNA POLYMERASE BETA WITH A DIDEOXY TERMINATED PRIMER AND 2'-DEOXYGUANOSINE 5'-BETA, GAMMA-METHYLENE TRIPHOSPHATE

Molecular Description:

Polymerase (DNA directed)
beta/DNA Complex
2'-DEOXY-5'-O-(HYDROXY{[HYDROXY(PHOSPHONOMETHYL)PHOSPHORYL]OXY}PHOSPHORYL)GUANOSINE

Deposited:

2006-10-18

Released:

2007-01-30

Structural Keywords:

B DOUBLE HELIX, NICKED

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sucato, C.A., Upton, T.G., Kashemirov, B.A., Batra, V.K., Martinek, V., Xiang, Y., Beard, W.A., Pedersen, L.C., Wilson, S.H., McKenna, C.E., Florian, J., Warshel, A., Goodman, M.F.
Modifying the beta,gamma Leaving-Group Bridging Oxygen Alters Nucleotide Incorporation Efficiency, Fidelity, and the Catalytic Mechanism of DNA Polymerase beta. 
Biochemistry, 46, pp. 461 - 471, 2007.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 50.7   b = 80.4   c = 55.5 (Ångstroms)

α = 90.0   β = 107.9   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.201 for 19344 reflections in the resolution range 22.36 to 2.2 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)

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Asymmetric Unit coordinates (pdb format, Unix compressed(.gz))

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Biological Assembly coordinates (pdb format) 1

Structure Factors (cif format)

Coordinates (pdb format, Unix compressed(.gz))

Coordinates (cif format, Unix compressed(.gz))

NMR Restraints (cif format, Unix compressed(.gz))

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