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NDB ID: PD0936    PDB ID: 2C7R     NAKB(beta): PD0936 


Title:

HHAI DNA METHYLTRANSFERASE (T250G MUTANT) COMPLEX WITH OLIGONUCLEOTIDE CONTAINING 2-AMINOPURINE AS A TARGET BASE (GPGC:GMGC) AND SAH

Molecular Description:

MODIFICATION METHYLASE HHAI (E.C.2.1.1.37)

Deposited:

2005-11-27

Released:

2005-12-14

Structural Keywords:

B DOUBLE HELIX, BULGES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Neely, R.K., Daujotyte, D., Grazulis, S., Magennis, S.W., Dryden, D.T.F., Klimasauskas, S., Jones, A.C.
Time-Resolved Fluorescence of 2-Aminopurine as a Probe of Base Flipping in M.HhaI-DNA Complexes. 
Nucleic Acids Res., 33, pp. 6953 - , 2005.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

H 3 2

Cell Constants:

a = 95.62   b = 95.62   c = 315.756 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.199 for 44234 reflections in the resolution range 29.5 to 1.9 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)