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NDB ID: PD0969    PDB ID: 2OPF 


Title:

CRYSTAL STRUCTURE OF THE DNA REPAIR ENZYME ENDONUCLEASE-VIII (NEI) FROM E. COLI (R252A) IN COMPLEX WITH AP-SITE CONTAINING DNA SUBSTRATE

Molecular Description:

Endonuclease VIII (E.C.3.2.2.-
E.C.4.2.99.18)/DNA Complex

Deposited:

2007-01-29

Released:

2008-02-26

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Golan, G., Zharkov, D.O., Grollman, A.P., Shoham, G.
Active site plasticity of endonuclease VIII: Conformational changes compensating for different substrates and mutations of critical residues
To be Published, , pp. - , 0.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 43 2 2

Cell Constants:

a = 73.556   b = 73.556   c = 170.113 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.24 for 36395 reflections in the resolution range 20.41 to 1.85 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)