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NDB ID: PD0998    PDB ID: 2UVR 


Title:

CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS

Molecular Description:

DNA POLYMERASE IV (E.C.2.7.7.7)

Deposited:

2007-03-13

Released:

2007-05-08

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Eoff, R.L., Irimia, A., Angel, K.C., Egli, M., Guengerich, F.P.
Hydrogen Bonding of 7,8-Dihydro-8-Oxodeoxyguanosine with a Charged Residue in the Little Finger Domain Determines Miscoding Events in Sulfolobus Solfataricus DNA Polymerase Dpo4. 
J.Biol.Chem., 282, pp. 19831 - , 2007.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 94.719   b = 104.197   c = 52.723 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.232 for 11732 reflections in the resolution range 46.07 to 2.9 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)