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NDB ID: PD1010    PDB ID: 2PY5 


Title:

PHI29 DNA POLYMERASE COMPLEXED WITH SINGLE-STRANDED DNA

Molecular Description:

Deposited:

2007-05-15

Released:

2007-07-17

Structural Keywords:

SINGLE STRAND

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Berman, A.J., Kamtekar, S., Goodman, J.L., Lazaro, J.M., de Vega, M., Blanco, L., Salas, M., Steitz, T.A.
Structures of phi29 DNA polymerase complexed with substrate: the mechanism of translocation in B-family polymerases 
Embo J., 26, pp. 3494 - 3505, 2007.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 54.195   b = 200.178   c = 66.989 (Ångstroms)

α = 90.0   β = 109.4   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.16547 for 163498 reflections in the resolution range 40.59 to 1.6 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)