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NDB ID: PD1062    PDB ID: 3BM3 


Title:

RESTRICTION ENDONUCLEASE PSPGI-SUBSTRATE DNA COMPLEX

Molecular Description:

PspGI restriction endonuclease/DNA Complex

Deposited:

2007-12-12

Released:

2008-09-16

Structural Keywords:

B DOUBLE HELIX, BULGES, FLIPPED-OUT BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Szczepanowski, R.H., Carpenter, M., Czapinska, H., Tamulaitis, G., Siksnys, V., Bhagwat, A., Bochtler, M.
A direct crystallographic demonstration that Type II restriction endonuclease PspGI flips nucleotides
To be Published, , pp. - , 0.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 46.884   b = 96.06   c = 127.069 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.171 for 63135 reflections in the resolution range 19.38 to 1.7 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)