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NDB ID: PD1183    PDB ID: 3EEO 


Title:

M. HHAI CO-CRYSTALLIZED WITH SYNTHETIC DSDNA CONTAINING A PROPANE DIOL IN PLACE OF THE DEOXYCYTIDINE RESIDUE TARGETED FOR METHYLATION.

Molecular Description:

Modification methylase HhaI (E.C.2.1.1.37)/DNA Complex

Deposited:

2008-09-05

Released:

2010-03-02

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Porta, J.C., Christman, J.K., Borgstahl, G.E.O.
M. HhaI co-crystallized with synthetic dsDNA containing a propane diol in place of the deoxycytidine residue targeted for methylation.
To be Published, , pp. - , 0.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

H 3 2

Cell Constants:

a = 95.479   b = 95.479   c = 318.353 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.23858 for 39593 reflections in the resolution range 40.99 to 1.94 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)