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NDB ID: PD1209    PDB ID: 3FC3 


Title:

CRYSTAL STRUCTURE OF THE BETA-BETA-ALPHA-ME TYPE II RESTRICTION ENDONUCLEASE HPY99I

Molecular Description:

Restriction endonuclease in complex with substrate DNA

Deposited:

2008-11-21

Released:

2009-03-31

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sokolowska, M., Czapinska, H., Bochtler, M.
Crystal structure of the beta beta alpha-Me type II restriction endonuclease Hpy99I with target DNA. 
Nucleic Acids Res., 37, pp. 3799 - 3810, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

H 3 2

Cell Constants:

a = 90.089   b = 90.089   c = 334.254 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.18212 for 51564 reflections in the resolution range 19.71 to 1.75 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)