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NDB ID: PDE014    PDB ID: 1RVA 


Title:

MG2+ BINDING TO THE ACTIVE SITE OF ECO RV ENDONUCLEASE: A CRYSTALLOGRAPHIC STUDY OF COMPLEXES WITH SUBSTRATE AND PRODUCT DNA AT 2 ANGSTROMS RESOLUTION

Molecular Description:

ECO RV (E.C.3.1.21.4)/DNA COMPLEX

Deposited:

1994-10-21

Released:

1995-01-26

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASE, KINKED

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Kostrewa, D., Winkler, F.K.
Mg2+ binding to the active site of EcoRV endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 A resolution. 
Biochemistry, 34, pp. 683 - 696, 1995.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 49.44   b = 50.15   c = 63.95 (Ångstroms)

α = 96.54   β = 109.05   γ = 108.11 (degrees)

Refinement:

The structure was refined using the X-PLOR program. The R value is 0.162 for 33333 reflections in the resolution range 20.0 to 2.0 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)