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NDB ID: PDE0142    PDB ID: 1A31 


Title:

HUMAN RECONSTITUTED DNA TOPOISOMERASE I IN COVALENT COMPLEX WITH A 22 BASE PAIR DNA DUPLEX

Molecular Description:

TOPOISOMERASE I (E.C.5.99.1.2)/DNA COMPLEX

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Redinbo, M.R., Stewart, L., Kuhn, P., Champoux, J.J., Hol, W.G.
Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA. 
Science, 279, pp. 1504 - 1513, 1998.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 72.0   b = 66.6   c = 71.8 (Ångstroms)

α = 90.0   β = 98.3   γ = 90.0 (degrees)

Refinement:

The structure was refined using the X-PLOR 3.1 program. The R value is 0.247 for 42786 reflections in the resolution range 20.0 to 2.1 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)