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NDB ID: PDE0146    PDB ID: 1BUA 


Title:

STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE

Molecular Description:

ENDONUCLEASE ECORV (E.C.3.1.21.4)/DNA COMPLEX

Deposited:

1998-09-03

Released:

1998-09-09

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Martin, A.M., Sam, M.D., Reich, N.O., Perona, J.J.
Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease. 
Nat.Struct.Biol., 6, pp. 269 - 276, 1999.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 50.3   b = 64.2   c = 49.4 (Ångstroms)

α = 109.3   β = 108.3   γ = 96.3 (degrees)

Refinement:

The structure was refined using the X-PLOR program. The R value is 0.0 for 22040 reflections in the resolution range 6.0 to 2.15 Ångstroms with Fobs >  1.0 sigma(Fobs) and with I >  0.0 sigma(I)