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NDB ID: PDR043    PDB ID: 2PUE 


Title:

CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES

Molecular Description:

Deposited:

1997-10-04

Released:

1998-05-06

Structural Keywords:

B DOUBLE HELIX, THE DNA IS KINKED 45 DEGREES AT THE CENTER

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Lu, F., Schumacher, M.A., Arvidson, D.N., Haldimann, A., Wanner, B.L., Zalkin, H., Brennan, R.G.
Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190. 
Biochemistry, 37, pp. 971 - 982, 1998.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 175.81   b = 95.17   c = 81.31 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the TNT program. The R value is 0.175 for 17561 reflections in the resolution range 10.0 to 2.7 Ångstroms with Fobs >  1.1 sigma(Fobs) and with I >  0.0 sigma(I)