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NDB ID: PH0008    PDB ID: 1S0V 


Title:

STRUCTURAL BASIS FOR SUBSTRATE SELECTION BY T7 RNA POLYMERASE

Molecular Description:

T7 RNA polymerase(E.C.2.7.7.6)/DNA/RNA Complex

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Temiakov, D., Patlan, V., Anikin, M., McAllister, W.T., Yokoyama, S., Vassylyev, D.G.
Structural basis for substrate selection by t7 RNA polymerase. 
Cell(Cambridge,Mass.), 116, pp. 381 - 391, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 81.133   b = 87.703   c = 206.531 (Ångstroms)

α = 91.93   β = 91.02   γ = 110.66 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.255 for 73174 reflections in the resolution range 40.0 to 3.2 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)