A Portal for Three-dimensional Structural Information about Nucleic Acids
As of 29-Jun-2016 number of released structures: 8332
Search for released structures

NDB ID: PH0011    PDB ID: 1SFO 


Title:

RNA POLYMERASE II STRAND SEPARATED ELONGATION COMPLEX

Molecular Description:

DNA-directed RNA polymerase II largest subunit (E.C.2.7.7.6)/DNA-directed RNA polymerase II 140 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 45 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 27 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 23 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 14.5 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 14.2 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 8.3 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 13.6 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 7.7 kDa polypeptide (E.C.2.7.7.6)/DNA/RNA Complex

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 10 protein sequences

Primary Citation:

Westover, K.D., Bushnell, D.A., Kornberg, R.D.
Structural Basis of Transcription: Separation of RNA from DNA by RNA Polymerase II 
Science, 303, pp. 1014 - 1016, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 167.076   b = 221.256   c = 193.685 (Ångstroms)

α = 90.0   β = 100.1   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.315 for 73591 reflections in the resolution range 39.86 to 3.61 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)