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NDB ID: PH0017    PDB ID: 1ZBL 


Title:

BACILLUS HALODURANS RNASE H CATALYTIC DOMAIN MUTANT D192N IN COMPLEX WITH 12-MER RNA/DNA HYBRID

Molecular Description:

ribonuclease H-related protein/RNA/DNA Complex

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Nowotny, M., Gaidamakov, S.A., Crouch, R.J., Yang, W.
Crystal Structures of RNase H Bound to an RNA/DNA Hybrid: Substrate Specificity and Metal-Dependent Catalysis. 
Cell(Cambridge,Mass.), 121, pp. 1005 - 1016, 2005.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 36.897   b = 44.983   c = 62.28 (Ångstroms)

α = 83.63   β = 89.9   γ = 65.69 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.217 for 15213 reflections in the resolution range 30.0 to 2.2 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)