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NDB ID: PH0024    PDB ID: 2G8V 


Title:

B. HALODURANS RNASE H CATALYTIC DOMAIN E188A MUTANT IN COMPLEX WITH MG2+ AND RNA/DNA HYBRID (REACTION PRODUCT)

Molecular Description:

RIBONUCLEASE H (E.C.3.1.26.4)/RNA/DNA complex

Structural Keywords:

RH DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Nowotny, M., Yang, W.
Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release. 
Embo J., 25, pp. 1924 - 1933, 2006.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 81.871   b = 36.809   c = 62.144 (Ångstroms)

α = 90.0   β = 97.17   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.0 for 14810 reflections in the resolution range 30.0 to 1.85 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  2.4 sigma(I)