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NDB ID: PH0026    PDB ID: 2HVR 


Title:

STRUCTURE OF T4 RNA LIGASE 2 WITH NICKED 5'-ADENYLATED NUCLEIC ACID DUPLEX CONTAINING A 3'-DEOXYRIBONUCLEOTIDE AT THE NICK

Molecular Description:

T4 RNA Ligase 2/DNA/RNA Complex
2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1
3-DIOL

Structural Keywords:

RH DOUBLE HELIX, BULGES

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Nandakumar, J., Shuman, S., Lima, C.D.
RNA Ligase Structures Reveal the Basis for RNA Specificity and Conformational Changes that Drive Ligation Forward. 
Cell(Cambridge,Mass.), 127, pp. 71 - 84, 2006.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 83.569   b = 106.16   c = 125.329 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.0 for 41156 reflections in the resolution range 19.92 to 2.45 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)