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NDB ID: PH0028    PDB ID: 2E2H 


Title:

RNA POLYMERASE II ELONGATION COMPLEX AT 5 MM MG2+ WITH GTP

Molecular Description:

DNA-directed RNA polymerase II largest subunit (E.C.2.7.7.6)/DNA-directed RNA polymerase II 140 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 45 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 27 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 23 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 14.5 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 14.2 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 8.3 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 13.6 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II
and III 7.7 kDa polypeptide (E.C.2.7.7.6)/DNA/RNA

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASES, NICKED

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 10 protein sequences

Primary Citation:

Wang, D., Bushnell, D.A., Westover, K.D., Kaplan, C.D., Kornberg, R.D.
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis 
Cell(Cambridge,Mass.), 127, pp. 941 - 954, 2006.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 171.108   b = 222.005   c = 195.163 (Ångstroms)

α = 90.0   β = 102.61   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2.0019 program. The R value is 0.29421 for 57635 reflections in the resolution range 45.08 to 3.95 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)