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NDB ID: PH0036    PDB ID: 2O5I 


Title:

CRYSTAL STRUCTURE OF THE T. THERMOPHILUS RNA POLYMERASE ELONGATION COMPLEX

Molecular Description:

DNA-directed RNA polymerase alpha chain
DNA-directed RNA polymerase beta chain
DNA-directed RNA polymerase beta' chain
DNA-directed RNA polymerase omega chain/DNA/RNA Complex

Structural Keywords:

RH DOUBLE HELIX, NICKED, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 4 protein sequences

Primary Citation:

Vassylyev, D.G., Vassylyeva, M.N., Perederina, A., Tahirov, T.H., Artsimovitch, I.
Structural basis for transcription elongation by bacterial RNA polymerase. 
Nature, 448, pp. 157 - 162, 2007.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 41

Cell Constants:

a = 156.211   b = 156.211   c = 499.229 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.0 program. The R value is 0.2402 for 347412 reflections in the resolution range 20.0 to 2.5 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)